Plasmid_Backbone

Part:BBa_K4284019:Design

Designed by: Zhang Xinchi   Group: iGEM22_PINGHE   (2022-08-11)


pET28a-EGFP-backbone


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 192
    Illegal XbaI site found at 962
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 192
    Illegal NotI site found at 165
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 192
    Illegal BglII site found at 1013
    Illegal BamHI site found at 198
    Illegal XhoI site found at 158
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 192
    Illegal XbaI site found at 962
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 192
    Illegal XbaI site found at 962
    Illegal NgoMIV site found at 1045
    Illegal NgoMIV site found at 2633
    Illegal NgoMIV site found at 2793
    Illegal NgoMIV site found at 5840
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 272
    Illegal SapI site found at 3713


Design Notes

N0


Source

N0

References